Fig. 2

ELS opens chromatin in experience-activated cells of the VTA. (a) Volcano plot of differentially accessible regions (DARs) within experience-activated cells. (DESeq2, p-value < 0.01 and |logFC|>1). DARs opened by ELS shown in red. (b) Average log₂(FoldChange) for actual and shuffled sample labels of the 100 lowest p-value regions comparing ELS and Std with each set of labels. Actual labels z-score = 1.96 vs. shuffled means (p = 0.02). (c) Volcano plot with log2(foldchange) shrinkage showing opening (red, +) and closing (blue, -) regions in activated population. (d) Example browser tracks of ATAC peaks opened by ELS in experience-activated cells, RPM-normalized (chr12:54877473–54877631–left, chr4:80149575–80150001–center, chr11:38084665–38084873–right). (e) Volcano plot of DARs within non-activated cells. (DESeq2, p-value < 0.01 and |logFC|>1). DARs opened by ELS shown in orange; closed by ELS shown in dark grey. (f) Enrichment or depletion (log₁₀ of observed over expected for each genomic feature in the mm39 genome) of DARs mapped to each genomic feature for DARs. Inset shows proportion of DARs found in putative cis-regulatory elements (> 200bb from the nearest TSS (red, 88%), within proximity to the TSS (light red, 9%), or at the TSS (white, 3%). (g) Top transcription factor motifs found in DARs.