Abstract
Rice-wheat system is a significant system for food production which generates a substantial amount of rice residues. Burning is a major challenge in Punjab and Haryana states of India that causes air pollution and greenhouse gases emission. The present investigation was aimed to identify the microbes and their potential for faster decomposition of rice residue with recommended rate of synthetic fertilizers for sustainably enhancing PAU Happy Seeder sown wheat production. The experimental findings revealed that generally 150 kg N ha− 1 (25% through farm yard manure and 75% through urea) (N6) with microbial spray of consortia (Aspergillus sp. + Delftia sp.) (M4) on in-situ rice residue after paddy harvest increased microbial viable cell counts i.e. total aerobic bacterial population (BP), fungal population (FP) and cellulose degrading bacteria population (CDBP), analysed using Scanning Electron Microscope (SEM) and Fourier Transform Infrared (FTIR) spectroscopy. The enrichment of microbial population led to better decomposition process of paddy straw that might have resulted to higher wheat productivity. Application of 150 kg N ha− 1 along with 3% urea spray (N5) resulted in substantial yield increase from 9.1 to 17.2% over 120 kg N ha− 1 (recommended dose of fertilizer - RDF) (N2) at Punjab Agricultural University, Ludhiana and University Seed Farm, Ladhowal. The interaction of N6M4 often producing the highest values and significantly influenced microbial viable cell counts at specific growth stages at both the location. The first principal component (Dim1) explains a major proportion of the variance (84.2 to 95.9%), while the second component (Dim2) contributes a smaller proportion (2.8 to 8.3%) indicating that Dim1 depicting the strong relationships among BP, FP and CDBP. Hence, Delftia sp. under N @150 kg ha− 1 regime increased the succeeding wheat yield and effectively faster decomposition of rice residue.
Data availability
The datasets generated and analysed during the current study are not publicly available due to confidentiality, but are available from the corresponding author on reasonable request.
References
Bhatt, R., Singh, P., Hossain, A. & Timsina, J. Rice-wheat system in the Northwest Indo-Gangetic plains of South asia: issues and technological interventions for increasing productivity and sustainability. Paddy Water Environ. 19 (3), 345–365. https://doi.org/10.1007/s10333-021-00846-7 (2021).
Memon, M. S. et al. The effects of tillage and straw incorporation on soil organic carbon status, rice crop productivity, and sustainability in the rice-wheat cropping system of Eastern China. Sustain 10, 961. https://doi.org/10.3390/su10040961 (2018).
Gupta, R. K. et al. Interactive effects of long term management of crop residue and phosphorus fertilization on wheat productivity and soil health in the rice-wheat. Sci. Rep. 14, 1399. https://doi.org/10.1038/s41598-024-51399-8 (2024).
Bhuvaneshwari, S., Hettiarachchi, H. & Meegoda, J. N. Crop residue burning in india: policy challenges and potential solutions. Int. J. Environ. Res. Publ Health. 16 (5), 832 (2019). 10.3390%2Fijerph16050832.
Meena, R. P. et al. Effect of rice residue retention and foliar application of K on water productivity and profitability of wheat in North West India. Agronomy 10, 434. https://doi.org/10.3390/agronomy10030434 (2020).
Lohan, S. K. et al. Burning issues of paddy residue management in north-west States of India. Renew. Sust Ener Rev. 81, 693–706. https://doi.org/10.1016/j.rser.2017.08.057 (2018).
Ye, J. et al. Denitrifying communities enriched with mixed nitrogen oxides preferentially reduce N2O under conditions of electron competition in waste water. Chem. Eng. J. 498, 155292. https://doi.org/10.1016/j.cej.2024.155292 (2024).
NPMCR (National Policy for Management of Crop Residues). Incorporation in Soil and Mulching Baling/Binder for Domestic/Industrial as Fuel Government of India Ministry of Agriculture Department of Agriculture & Cooperation, 2023. Available online: (2023). http://agricoop.nic.in/sites/default/files/NPMCR_1.pdf
Kumar, A. et al. Effect of paddy straw burning on soil microbial dynamics in sandy loam soil of Indo-Gangetic plains. Environ. Tech. Innov. 16, 100469. https://doi.org/10.1016/j.eti.2019.100469 (2019).
Sidhu, H. S. et al. Development and evaluation of the turbo happy seeder for sowing wheat into heavy rice residues in NW India. Field Crop Res. 184, 201–212. https://doi.org/10.1016/j.fcr.2015.07.02 (2015).
Matthews, S. Structural changes of rice straw pre-treated with Paenibacillus and Aspergillus fumigates. Inter J. Agri Food Res. 5, 1–8 (2016).
Shukla, L., Suman, A., Verma, P., Yadav, A. N. & Saxena, A. K. Syntrophic microbial system for ex-situ degradation of paddy straw at low temperature under controlled and natural environment. J. Appl. Biol. Biotechnol. 4, 30–37. https://doi.org/10.7324/JABB.2016.40205 (2016).
Choudhary, M. et al. Crop residue degradation by fungi isolated from conservation agriculture fields under rice-wheat system of North-West India. Int. J. Recyc Org. Waste Agric. 5, 349–360. https://doi.org/10.1007/s40093-016-0145-3 (2016).
Dash, P. K. et al. Efficient lignin decomposing microbial consortium to hasten rice-straw composting with moderate GHGs fluxes. Waste Biomass Valorization. 13 (1), 481–496. https://doi.org/10.1007/s12649-021-01508-9 (2022).
Wang, X. et al. The effects of mulch and nitrogen fertilizer on the soil environment of crop plants. Adv. Agron. 153, 122–173. https://doi.org/10.1016/bs.agron.2018.08.003 (2018).
Piper, C. S. Soil and Plant Analysis (International Science, 1966).
Richards, L. A. Diagnosis and Improvement of Saline and Alkali Soils (In: Agriculture Hand Book No. USDA, 1954).
Jackson, M. L. Estimation of phosphorous content. Soil. Chem. Anal. 2, 134–182 (1967).
Walkley, A. & Black, C. A. An examination of wet acid method for determining soil organic matter and a proposed modification of the chromic acid Titration method. Soil. Sci. 37, 29–38 (1934).
Subbiah, B. V. & Asija, G. L. A rapid procedure for the Estimation of available nitrogen in soils. Curr. Sci. 25, 259–260. https://doi.org/10.1007/BF01358352 (1956).
Olsen, S. R., Cole, C. V., Waternade, F. S. & Dean, L. A. Estimation of available phosphorus in soil by extraction with sodium bicarbonate. USDA Circ. 939, 1–19 (1954).
Anonymous Package and practices for crops of Punjab rabi 2023-24. Punjab Agricultural University, Ludhiana, Punjab, India. 1–31 https://pau.edu/content/ccil/pf/pp_rabi.pdf (2024).
Tondey, M. et al. A comparative evaluation of the effects of seed invigoration treatments with precursor zinc salt and nano-sized zinc oxide (ZnO) particles on vegetative growth, grain yield, and quality characteristics of Zea Mays. J. Anal. Sci. Technol. 13 (1), 1–14. https://doi.org/10.1186/s40543-022-00346-1 (2022).
Wright, H. D. The importance of adequate reduction of peptone in the Preparation of media for the Pneumococcus and other organisms. J. Path Bact. 37, 257–282. https://doi.org/10.5555/19342700145 (1933). https://www.cabidigitallibrary.org/doi/full/
Subba-Rao, N. S. Soil Microorganisms and Plant Growth (Oxford and IBH Publishing Co., 1977).
Sidhu, A. S., Kang, J. S. & Kingra, P. K. Study of microclimate and heat use efficiency of happy seeder sown wheat (Triticum aestivum L.) by using rice residue and nitrogen management practices. J. Agric. Phys. 20 (1), 30–39 (2020).
Kesarwani, A. et al. -situ rice residue management under rice-wheat cropping system and their influence on wheat productivity. J. Pharmacogn Phytochem. 6, 1422–1425 (2017).
Kharia, S. K. et al. Tillage and rice straw management affect soil enzyme activities and chemical properties after three years of conservation agriculture based rice-wheat system in North-western India. Int. J. Plant. Soil. Sci. 15, 1–13. https://doi.org/10.9734/IJPSS/2017/33494 (2017).
Verma, G. & Kaur, S. Effect of nitrogen levels alone and in combination with farm yard manure, potassium and phosphorus on grain yield. Int. J. Agric. Sci. 8, 1974–1975. https://doi.org/10.1007/BF01358352 (2016).
Kumar, A. et al. Rice residue management in wheat under different tillage practices and nitrogen doses. Annals Agric. Res. 37, 49–55 (2016). https://epubs.icar.org.in/index.php/AAR/article/view/57885
Blanchet, G., Gavazov, K., Bragazza, L. & Sinaj, S. Responses of soil properties and crop yields to different inorganic and organic amendments in a Swiss conventional farming system. Agric. Ecosys Environ. 230, 116–126. https://doi.org/10.1016/j.agee.2016.05.032 (2016).
Jin, Z. et al. Effect of straw returning on soil organic carbon in rice-wheat rotation system: A review. Food Ener Secur. 9 (2), e200. https://doi.org/10.1002/fes3.200 (2020).
Wang, L. et al. Inclusion of microbial inoculants with straw mulch enhances grain yields from rice fields in central China. Food Ener Secur. 9 (4), 230. https://doi.org/10.1002/fes3.230 (2020).
Bhagat, P. & Gosal, S. K. Long term application of rice straw and nitrogen fertilizer affects soil health and microbial communities. Chem. Sci. Rev. Lett. 7, 586–593 (2018).
Yao, Y. et al. Methane recovery from anaerobic digestion of urea-pretreated wheat straw. Renew Ener. 115, 139–148 https://doi.org/10.1016/j.renene.2017.08.0388 (2024). Denitrifying communities enriched with mixed nitrogen oxides preferentially reduce N2O under conditions of electron competition in waste water. Chem Eng J. 498, 155292. https://doi.org/10.1016/j.cej.2024.155292 (2018).
Chen, Y. et al. Application of Fenton pretreatment on the degradation of rice straw by mixed culture of Phanerochaete Chrysosporium and Aspergillus Niger. Indust Crops Prod. 112, 290–295. https://doi.org/10.1016/j.indcrop.2017.12.005 (2018).
Kaur, H. Pretreatment of paddy straw by Delftia sp. for enhancing biogas production. M.Sc. thesis. Punjab Agricultural University, Ludhiana, Punjab, India. (2018).
Tsegaye, B., Balomajumder, C. & Roy, P. Biodelignification and hydrolysis of rice straw by novel bacteria isolated from wood feeding termite. Biotechnol 8, 1–11. https://doi.org/10.1007/s13205-018-1471-0 (2018).
Vu, N. D., Tran, H. T., Bui, N. D., Vu, C. D. & Nguyen, H. V. Lignin and cellulose extraction from vietnam’s rice straw using Ultrasound-assisted Akaline treatment method. Int. J. Polym. Sci. 2017 (1), 1–8. https://doi.org/10.1155/2017/1063695 (2017).
Yin, C. et al. Valorization of rice straw via hydrotropic lignin extraction and its characterization. Molecules 26 (14), 4123. https://doi.org/10.3390/molecules26144123 (2021).
Wei, L., Ma, F. & Du, C. Application of FTIR-PAS in rapid assessment of rice quality under climate change conditions. Foods 10 (1), 159. https://doi.org/10.3390/foods10010159 (2021).
Remli, N. A., Shah, U. K., Mohamad, R. & Abd-Aziz, S. Effects of chemical and thermal pretreatments on the enzymatic saccharification of rice straw for sugars production. BioRes 9 (1), 510–522. https://doi.org/10.15376/biores.9.1.510-522 (2014).
Varol, E. A. & Mutlu, U. TGA-FTIR analysis of biomass samples based on the thermal decomposition behavior of hemicellulose, cellulose, and lignin. Energies 16 (9), 3674. https://doi.org/10.3390/en16093674 (2023).
Ahuja, D., Kaushika, A. & Chauhan, G. S. Fractionation and physicochemical characterization of lignin from waste jute bags: effect of process parameters on yield and thermal degradation. Int. J. Biol. Macromol. 97, 403–410 (2017).
Acknowledgements
The Jawaharlal Nehru Scholarships for Doctoral Studies and Punjab Agricultural University, Ludhiana, are gratefully acknowledged.
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RSK: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Supervision, Validation, Visualization, Writing-original draft, Writing - Review & editing, JS: Conceptualization, Formal analysis, Investigation, Methodology, Writing - Review & editing, AK: Conceptualization, Methodology, Supervision, Writing - Review & editing, KBS: Conceptualization, Investigation, Supervision, Writing - Review & editing, RS: Conceptualization, Writing - Review & editing, IS: Conceptualization, Writing - Review & editing, AP: Conceptualization, Writing - Review & editing.
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Khedwal, R.S., Singh, J., Kalia, A. et al. Spectroscopy based analysis of rice residue driven by microbial decomposition and nitrogen management under zero till wheat in Northern India. Sci Rep (2026). https://doi.org/10.1038/s41598-025-25793-9
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DOI: https://doi.org/10.1038/s41598-025-25793-9
