Table 1 Pentaplex qPCR primers and TaqMan-probes# sequences used in this study.
Pathogens | Gene | Primers/probes | Sequence | Product size |
|---|---|---|---|---|
Toxoplasma gondii | G3PDH | GZQTgG3PDH-F | 5′ - TTGCATAGGTTGCAGTCACTGA − 3′ | 78 bp |
GZQTgG3PDH-R | 5′ - ACCAAAGTTGCACAGATACTCATGA − 3′ | |||
GZQTgG3PDH-P | 5′- (FAM) - CCCCTCTGCTGGCGAAAAGTGAAA - (QSY) − 3′ | |||
Neospora caninum | NC5 | GZQNcNC5-F | 5′ - GCGGACGGGTCGTTGTT − 3′ | 75 bp |
GZQNcNC5-R | 5′ - CCGTTCACACACTATAGCCACAAA − 3′ | |||
GZQNcNC5-P | 5′ - (VIC) - CCTGCGGCAGCAAGGCTCCTT - (QSY) − 3′ | |||
Eimeria stiedai | ADF | GZQEsADF-F | 5′ - TCATCCTCAAGATTGACCACGAT − 3′ | 91 bp |
GZQEsADF-R | 5′ - TCACTAGCTGGAAGTTCCTTTGTG − 3′ | |||
GZQEsADF-P | 5 ′- (ABY) - AAGGGCACTGGTGACGCCTCAACTC - (QSY) − 3′ | |||
Giardia lamblia | GDH | GZQGlGDH-F | 5′ - GGGCGGCTCCGACTTT − 3′ | 62 bp |
GZQGlGDH-R | 5′ - ACTGGCAGAAGCGCATGAC − 3′ | |||
GZQGlGDH-P | 5′ - (JUN) - ACCCAAAGGGCAAGTCCGACAACG - (QSY) − 3′ | |||
Trypanosoma evansi | COX1 | GZQTeCOX1-F | 5′ - GGGTGACTTAGCCCATGCA − 3′ | 64 bp |
GZQTeCOX1-R | 5′ - CCCACACAATTGAAGAGAGAAAATAG − 3′ | |||
GZQTeCOX1-P | 5′ - (CY5) - AGCCTCGGGTGATC - (MGB) − 3′ |
