Table 4 Repeatability and reproducibility of the TaqMan-probe-based pentaplex qPCR assay for simultaneously detecting Toxoplasma gondii, Neospora caninum, Eimeria stiedai, Giardia lamblia and Trypanosoma evansi.

Toxoplasma gondii

1 × 10⁷ copies/µL

14.86 ± 0.03

0.20%

14.44 ± 0.09

0.67%

1 × 10⁶ copies/µL

17.93 ± 0.01

0.07%

17.81 ± 0.13

0.75%

1 × 10⁵ copies/µL

21.13 ± 0.05

0.25%

21.11 ± 0.08

0.40%

1 × 10⁴ copies/µL

24.49 ± 0.14

0.59%

24.47 ± 0.13

0.54%

1 × 10³ copies/µL

27.83 ± 0.10

0.37%

27.95 ± 0.17

0.63%

Neospora caninum

1 × 10⁷ copies/µL

17.07 ± 0.03

0.17%

16.88 ± 0.05

0.31%

1 × 10⁶ copies/µL

20.07 ± 0.09

0.45%

20.13 ± 0.16

0.80%

1 × 10⁵ copies/µL

23.58 ± 0.17

0.75%

23.60 ± 0.05

0.24%

1 × 10⁴ copies/µL

26.77 ± 0.12

0.46%

26.47 ± 0.06

0.22%

1 × 10³ copies/µL

29.93 ± 0.02

0.07%

29.91 ± 0.20

0.67%

Eimeria stiedai

1 × 10⁷ copies/µL

14.88 ± 0.31

2.10%

14.86 ± 0.22

1.49%

1 × 10⁶ copies/µL

18.22 ± 0.14

0.76%

18.27 ± 0.12

0.69%

1 × 10⁵ copies/µL

21.55 ± 0.04

0.19%

21.61 ± 0.10

0.47%

1 × 10⁴ copies/µL

24.93 ± 0.21

0.87%

25.06 ± 0.11

0.46%

1 × 10³ copies/µL

27.14 ± 0.28

1.03%

28.10 ± 0.22

0.79%

Giardia lamblia

1 × 10⁷ copies/µL

14.03 ± 0.20

1.43%

14.86 ± 0.31

2.13%

1 × 10⁶ copies/µL

18.39 ± 0.05

0.28%

18.36 ± 0.16

0.91%

1 × 10⁵ copies/µL

21.76 ± 0.23

1.07%

21.72 ± 0.16

0.75%

1 × 10⁴ copies/µL

25.08 ± 0.13

0.53%

25.03 ± 0.18

0.75%

1 × 10³ copies/µL

28.08 ± 0.11

0.40%

28.52 ± 0.14

0.50%

Trypanosoma evansi

1 × 10⁷ copies/µL

17.11 ± 0.16

0.95%

17.02 ± 0.15

0.89%

1 × 10⁶ copies/µL

20.31 ± 0.06

0.33%

20.21 ± 0.14

0.71%

1 × 10⁵ copies/µL

23.22 ± 0.20

0.89%

23.75 ± 0.17

0.71%

1 × 10⁴ copies/µL

26.18 ± 0.24

0.93%

26.52 ± 0.07

0.26%

1 × 10³ copies/µL

29.51 ± 0.14

0.47%

28.41 ± 0.20

0.71%