Table 5 Anti-interference test of the TaqMan-probe-based pentaplex qPCR assay for simultaneously detecting Toxoplasma gondii, Neospora caninum, Eimeria stiedai, Giardia lamblia and Trypanosoma evansi.
Item | Toxoplasma gondii | Neospora caninum | Eimeria stiedai | Giardia lamblia | Trypanosoma evansi |
|---|---|---|---|---|---|
①| 1 × 108 copies/µL | 1 × 102 copies/µL | 1 × 102 copies/µL | 1 × 102 copies/µL | 1 × 102 copies/µL |
Cq value | 11.40 | 31.90 | 30.13 | 30.15 | 31.27 |
② | 1 × 102 copies/µL | 1 × 108 copies/µL | 1 × 102 copies/µL | 1 × 102 copies/µL | 1 × 102 copies/µL |
Cq value | 30.83 | 15.93 | 30.92 | 30.72 | 31.39 |
③ | 1 × 102 copies/µL | 1 × 102 copies/µL | 1 × 108 copies/µL | 1 × 102 copies/µL | 1 × 102 copies/µL |
Cq value | 30.24 | 31.83 | 11.47 | 30.27 | 31.03 |
④ | 1 × 102 copies/µL | 1 × 102 copies/µL | 1 × 102 copies/µL | 1 × 108 copies/µL | 1 × 102 copies/µL |
Cq value | 30.61 | 31.61 | 30.73 | 11.44 | 31.04 |
⑤ | 1 × 102 copies/µL | 1 × 102 copies/µL | 1 × 102 copies/µL | 1 × 102 copies/µL | 1 × 108 copies/µL |
Cq value | 30.44 | 31.96 | 30.41 | 30.07 | 14.29 |
⑥ | 1 × 102 copies/µL | 1 × 108 copies/µL | 1 × 108 copies/µL | 1 × 108 copies/µL | 1 × 108 copies/µL |
Cq value | 30.18 | 15.94 | 11.31 | 11.40 | 14.78 |
⑦ | 1 × 108 copies/µL | 1 × 102 copies/µL | 1 × 108 copies/µL | 1 × 108 copies/µL | 1 × 108 copies/µL |
Cq value | 11.81 | 31.95 | 11.34 | 11.40 | 14.60 |
⑧ | 1 × 108 copies/µL | 1 × 108 copies/µL | 1 × 102 copies/µL | 1 × 108 copies/µL | 1 × 108 copies/µL |
Cq value | 11.25 | 15.91 | 30.26 | 11.41 | 14.99 |
⑨ | 1 × 108 copies/µL | 1 × 108 copies/µL | 1 × 108 copies/µL | 1 × 102 copies/µL | 1 × 108 copies/µL |
Cq value | 11.25 | 15.86 | 11.15 | 30.02 | 14.86 |
⑩ | 1 × 108 copies/µL | 1 × 108 copies/µL | 1 × 108 copies/µL | 1 × 108 copies/µL | 1 × 102 copies/µL |
Cq value | 11.93 | 15.96 | 11.45 | 11.49 | 31.28 |
