Fig. 4
Representative immunohistochemical images of PCNA, γH2AX and cleaved caspase-3, respectively, in the normal colon (a, f, k), PC colon after AOM/DSS induced CRC (b, g, l), colon after IRN-monotherapy (c, h, m), colon after TG-monotherapy (d, i, n), and colon after administration of both IRN + TG (e, j, o); IHC scoring of colon for (p) PCNA positive cells per HPF (***p < 0.001, when NC v/s PC; **p < 0.01, when PC v/s IRN and PC v/s TG; and ***p < 0.001, when PC v/s IRN + TG), (q) γH2AX positive cells per HPF (*p < 0.05, when NC v/s PC and PC v/s TG; **p < 0.01, when PC v/s IRN; and ***p < 0.001, when PC v/s IRN + TG), and (r) cleaved caspase-3 positive cells per HPF (*p < 0.05, when NC v/s PC; **p < 0.01, when PC v/s IRN; and ***p < 0.001, when PC v/s TG, and PC v/s IRN + TG). One way ANOVA followed by post hoc Tukey test was performed for comparison among the groups.
