Table 2 Primer sequences.
Exon | Name | Sequence (5’-3’) | Modifications | Target |
|---|---|---|---|---|
Primers for Exon amplification | ||||
Exon 19 | Tag1-19del | TCCCGCGAAATTAATACGACAAGTTAAAATTCCCGTCGCTATC | Â | EGFR exon 19 |
| Â | Tag2-19del | GCTGGAGCTCTGCAGCTAGACCCCCACACAGCAAAG | Â | EGFR exon 19 |
Exon 20 | Tag1-T790M | TCCCGCGAAATTAATACGACGCATCTGCCTCACCTCCAC | Â | EGFR exon 20 |
| Â | Tag2-T790M | GCTGGAGCTCTGCAGCTAAGCAGGTACTGGGAGCCAAT | Â | EGFR exon 20 |
Exon 21 | Tag1-L858R | TCCCGCGAAATTAATACGACAGCCAGGAACGTACTGGTGA | Â | EGFR exon 21 |
| Â | Tag2-L858R | GCTGGAGCTCTGCAGCTATGCCTCCTTCTGCATGGTAT | Â | EGFR exon 21 |
Emulsion PCR primers | ||||
| Â | Tag1-F | TCCCGCGAAATTAATACGAC | Â | Â |
| Â | Tag2-R | GCTGGAGCTCTGCAGCTA | Â | Â |
Hybridization probe for BEAMing | ||||
Exon 19 | 19del_35_49_Cy5 | GGAGATGTTTTGATAGCG | 5′ Cy5 | exon 19 E746-A750del |
|  | 19del_36_50_Cy5 | CGGAGATGTCTTGATAGC | 5′ Cy5 | exon 19 E746-A750del |
|  | 19del_40_57 Cy5 | TGGCTTTCGATTCCTTGA | 5′ Cy5 | exon 19 L747-S752del.P753S |
|  | 19del_AATTCC_ Cy5 | TGTTGCTTCTCTTGGAATT | 5′ Cy5 | exon 19 E746-L747del.IP |
|  | 19del_36_55_T_ Cy5 | GCTTTCGGAACCTTGATAG | 5′ Cy5 | exon 19 L747-S752del. E746V |
|  | 19del_35_53_ACT_ Cy5 | GGAGAAGTTTTGATAGCG | 5′ Cy5 | exon 19 K745-E749del.A750K |
|  | 19del_39_56_CAG_ Cy5 | TTTCGGCTGTTCCTTGAT | 5′ Cy5 | exon 19 L747-T751del.S752Q |
|  | 19del_39_48_C_ Cy5 | GAGATGTTGGTTCCTTGAT | 5′ Cy5 | exon 19 L747-E749del.A750P |
|  | 19del_WT_488 | TGTTGCTTCTCTTAATTCC | 5′ Alexa488 | exon 19 wild-type control |
Exon 20 | T790M_Mut- Cy5 | atgagctgcAtgatgag | 5′ Cy5 | T790M mutation |
|  | T790M_WT-488 | tgagctgcGtgatgag | 5′ Alexa488 | Wild-type control for T790M |
Exon 21 | L858R_Mut_LNA_ Cy5 | gtttggccCgcccaaaat | 5′ Cy5 | L858R mutation |
|  | L858R_WT_LNA_488 | gtttggccAgcccaaaat | 5′ Alexa488 | Wild-type control for L858R |
|  | L861Q_Mut_Cy5 | cacccagcTgtttggcc | 5′ Cy5 | L861Q mutation |
|  | L861Q_WT_488 | cacccagcAgtttggcc | 5′ Alexa488 | Wild-type control for L861Q |
