Fig. 1

Positioning and relevant anatomy. Figure 1A: The mouse was positioned in dorsal recumbent position, with its nose secured in a tubing apparatus for continuous isoflurane delivery. Tissue retractors were positioned adjacent to the abdomen in preparation for the procedure. Figure 1B: The location and planned size of the incision is shown, measuring approximately 2 cm, as indicated by a ruler held next to the ink mark. Figure 1C: The opening through the linea alba is depicted, with entry into the abdominal compartment. Elevation of the abdominal musculature while expanding the opening was crucial for preventing bowel injury. Figure 1D: Relevant anatomical structures are visualized upon retracting the abdominal wall.