Fig. 3 | Scientific Reports

Fig. 3

From: Non-thermal plasma as promising anti-cancer therapy against bladder cancer by inducing DNA damage and cell cycle arrest

Fig. 3

Effect of PAS on cell death and phosphoproteome. (A) Effect of 1 h of 20% PAS exposure on the induction of cell death. (B) The effect of 1 h 20% PAS exposure on clonogenic cell survival. *p < 0.05; **p < 0.01. (C) Western blot of cleaved caspase 3 and cleaved cathepsin B after 1 h 20% PAS exposure and its quantification. *p < 0.05. All datapoints show an average of 3 independent experiments. (D) Supervised heatmap (p < 0.05) of phosphopeptides measured using LC–MS/MS based proteomics, 1 h after 20% PAS exposure. (E) Inka-ratio, calculated by dividing the Inka score of PAS treated cells vs untreated cells, showing an increased in various cell cycle related kinases. (F) String-network of significantly (p < 0.05) up and downregulated phosphoproteins. All datapoints show an average of 3 independent experiments.

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