Table 1 Endogenous peptides sequenced from the radial nerve cords of H. Scabra .

From: The neuropeptidomes of the sea cucumbers Stichopus cf. horrens and Holothuria scabra

NEUROPEPTIDE PRECURSOR SEQUENCE

PEPTIDE SEQUENCE

PRECURSOR DATABASE MATCH 

SEQUENCE LENGTH (# of Aas)

MASS ERROR (in ppm)

Molecular Weight (in Da)

m/z,  CHARGE STATE

RETENTION TIME (in mins)

POST TRANSLATIONAL MODIFICATIONS

PUTATIVE ANNOTATION

MSTFYRIGQKYCPDGRKFESESVEKAKHSYQVIFIRNMSIFTFSENAQLNEISADGEEDKRQYFAGKRQLPGGDEYEDKRQYFTGKRQLPGGDAGDVEDKRQYFAGKRQLPGGDAGDFEDKRQYFAGKRQLPGGKDYEDKRQYFNGKRQYFAGTREFLGVGQDNTEDKRQYFAGKRQLPGGQEYEDKRQYFAGKRQLPGGEAGDLEDKRQYFAGKRQLPGGNEFDHKRQYFAGKRQLPGGDVKDFADKRQYFAGKRQLPGGEAGDLEDKRQYFAGKRQLPGGDAYEDKRQYFVGKRQLPGGEEYEDKRQYFSGKRMQTDAELNDETQLFSEKREYEGDAEHEDKRQYFAEKRQLPGGDVEDGMMAKRQHFIGKRQLPEGWVNVNSDDKRQYFVGKRQLPGGDFEDLADKRQYFVGKRQLPGGQTDADYIKRQFFHGKRQLPGGQTMNTNRQYFAQKRQLPAGPWAFWEGKRLQDALNDALRPAVVYGGYHKRITDTIELKINVTLQRYRTGLLSDYCKRKAITQEIPGLEYAGFSCPTLLEEKCQDLMKFYEGPSPAFKRHDYVQFYRQLAH

QLPAGPWAFWEGKRLQDALNDALRPAVVYGGYH

WNHF01002782.1.g12687 b

33

3.53

3677.84

920.47,  4+

7.44

Gln -> pyro-Glu

TRH-type Peptide

MKTSWVPITLCIFCYLLATVSAASISRPSAELDLSRQYPELYEYILRQFSADQPMEEKRRMGGCGDFSGCASLKAGRDLVRAMLRQPSKFGS

ASISRPSAELDLSRQYPELYEYILRQFSADQPMEE

HsCCTP-short a

35

2.75

4130.98

1033.75,  4+

7.07

None

Calcitonin-type peptide

MGDSRALRMGPKGPKQRYVSCVHRISIMHDRHKREYKHAYTCYRDLSFDAHNPTHLMLRDCGAEDKRFGNSAMLDPLSQSLMSGKREEDKRFGNYPMDPLSQSLMGKRSQDKRFGNYPMDPLSQSLMGKRSEEKRFGNFPMDPLSQSLMGKRSQDKRFGNYPMDPLSQSLMGKRSEEKRFGNYPMDPLSQSLMGKRSEEKRFGNYPMDPLSQSLMGKRNQEKRFGNYPMDPLSQSLMGRKRSAEKRFGNFPMDPLSQSLMAGGGRR

FGNYPMDPLSQSLM

WNHF01003549.1.g48745 b

14

2

1597.72

799.87,  2+

6.40

Amidated (C-term)

Pedal peptide-type neuropeptide

FGNFPMDPLSQSLMAGG

17

0.77

1766.81

884.41,  2+

6.60

Amidated (C-term)

 

MHKLLVLIASLLVILLGLEAVTEALPLTDIPQDEQYTDLELLDNVNEYVKRISDNDFAQRMSDHITQLARNKALRNRQRLALKAGKRSSMMDDTVDKRNLSQNDISQSRAQYMNKMLAYHMMSQLLGNAGRR

NLSQNDLSQGRSNYINQLLAYQKWSQLMGAA

Pigment-dsipersing factor (PDF)  a

31

2.18

3509.74

1170.92,  3+

6.51

Amidated (C-term)

Pigment-dispersing Factor-like peptide

MKNLCTAVMTTLTVVLLFVMITEAIPVADDNQELLERIARSALDNPTKEEAFRQLFYEYLLELQQQQDAEDPIYASKRLSGLPGIDSISMISKA

SALDNPTKEEAFRQLFYEYLLELQQQQDAED

GHHS01352850.1b

31

1.86

3730.76

1244.59,  3+

6.88

None

Neuropeptide 40

SALDNPTKEEAFRQLFYEYLLELQQQQDAEDPIYA

35

2.59

4174.99

1392.67,  3+

6.85

None

 

MKAYQILVPAVLCVLAAILARTEADDELRILNNRLYELTKELEERMREQHMEDADLIFADGQDKRAGVKTLSRQWSPLLGQTGIAFGKRTDDIDRAMSQSDHRAKKTRARTMFGHSALGFGKRSEYIPQDIWDLQNAANDLETFEETPVKKGMGFTGNTGILLGKRHADDAEE

TLSRQWSPLLGQTGIAF

 L-type SALMFamide a

17

1.43

1698.92

850.47,  2+

6.59

Amidated (C-term)

L-type SALMFamide precursor

AMGFTGNTGILL

12

1.43

1192.63

1193.63,  1+

6.17

Amidated (C-term)

MAISRILTTLAVLFLTLLVVTVWSYPLVNQKESQEGLYNDLFHALASKVNYGTPGSRNRHPFVDGFGSPHYSGSLQGTQDLRWNPQQLKMVKALQDAKEDVGKRDYNDLGFFFGKRANSDDANMQRQSRDYGDLGMFFGKRSGNDVTDDRDFEDFAA

DYGDLGFFF

WNHF01003000.1.g.27376 b

9

0.66

1078.48

1079.48, 1+

7.37

Amidated (C-term)

Cholecystokinin-type precursor 1

MIKMAEKLLLCLVVAAULVCSSMYVTDDQDDLSPLLLOMENERMNEMEMLADTLKENGPIRYRNNYAALPPMPWLMNNKR

NGPIRYRNNYAALPPMPWLMNN

GN-19 a

22

3.81

2601.26

868.09, 3+

6.36

None

GN19

DTKLSTIMNVHEIFLTFACLFMALLCVTTTPLALDDEMLDRELRDDQLFEEGLEDQMPMYRRTDAADDEFVRDAKAAQWNKNFMGISSLDNSIKGFKSHSVRCRSNDVCKYAGKKMFRCKCNSLQYCVADTPMSNAHCKYSAVGFVYKQ

TPLALDDEMLDREL

WNHF01002781.1.g12434 b

14

3.89

1629.79

815.90, 2+

5.95

None

Hypothetical Protein_Putative Novel HS Neuropeptide Precursor 1

MNSMTVIVLCCLFATLAIGTVNAVQHFEYLEVPVQEEKVPLFEKGEDLSKSRLRRASCKQLDIIPKGLRIRNPFSIGSGRTAQVAENICKKASQKLRNLALRNAYGKQTRLNKLIVSTKAAVQRQTPDTYTSTGVLNAMDKSYKRELNGCFKRKLRKLDNLISNDTFLKGLHEAKHPFLTNKFPKKMTSNTQPFRSSNFMNLSDYTLSKNEEDVLQNSLQFCPSRPLDPAGLCYDFSDFVRRIRLKEYFNEDLPPQAPNNLPIHKRSEWTPPEGRNYHIDKFVKTARSHLDSFLTEQSKQKIPLNLPLAQAQAIKKLRRNTNIVIRPADKGGATTILNTADYIKEAEQQLSNDHFYKLLHDDPSGSFSNKVRTLCNFLSEDGAEIVKELIPSNPRLGIFYTLPKIHKLTQLLATHLPPNSAPFDIENLSKEQIRTLAVEHRILPPSRPIISSIGTLTEHLSGQCLRIKRICSDPSDFDEHISKLTGYFLSAGYPLSIIKRQIHRAKKRSRSELLEYRIKSPSTRIPFVTSYHPIVKRLMGTLKKDFEFVKEDVSLLTTFNQPPLLAFRQPPNLKMLLTSSRLPADRKTFGGNAGFRGRWGPWRAMSYKPFSVFRHTVEKMKISEQLFLKQYWQYLEDNQGGHGDSGGRGGGGGDGGGGGDGGRGGGRGGGGRGSNGTPGGRGQDGQYGNGLRVGVEKEGKTVIMVDYEVVSMEEEEVEEGKMEMIEVEVKLLVIWKTWTNLSYRLLDRGRMYE

HFEYLEVPVQEEKVPLFE

WNHF01003421.1.g45331 b

18

4.45

2231.11

744.71, 3+

6.06

None

Hypothetical Protein

VQHFEYLEVPVQEEKVPLFE

20

1.7

2458.24

1230.13, 2+

6.12

None

MDFCPISWHNAETETNLKLKFTVDINSLVWGSRRKHQKSTMSLSQGTVTDITIPGPGPFGFRLAGGKDFGMPLHISKVTAGGKAQRANLREKDVILMINGQDIAACTHLDAQNKIKASFHEDLHLRTQRGDSRVWNPVSRDDSGNFKVSLKANQDQEFLHPGSKSNFTPKGYGSPQPPAPSAVPPPVGRGSAQVVHLQFNSPAGLYSEQNIAETFKGQTAGMIDNSMGNGEYQPEPYQPLRDTGNSPKPVAHQASPVSTRPVGMRSVKPPSSSRPSQPSQQVRDLPNCAVCESNIMPSPTPPTYSKPTQRSPQGYGSPQSYGNPQSYGNPQSYGNP

ITIPGPGPFGFRL

GHHS01371798.1 c

13

4.72

1370.76

686.39, 2+

6.74

None

Hypothetical Protein

-

ASGAPLP

WNHF01002742.1.g9341   b

7

2.66

653.34

654.33, 1+

3.88

N-term Acetylation

Putative myosin heavy chain

-

SEDPEQFL

WNFH01002041.1.g8338 b

8

0.76

1005.43

1006.44, 1+

5.78

N-term Acetylation

Putative myosin heavy chain

-

PFGYLFR

WNFH01003365.1.g42937 b

7

3.69

898.47

450.24, 2+

5.63

None

Collagen alpha-1 (XXVII)

-

FAGDDAPRAVFP

WNHF01003172.1.g35899 b

12

0.46

1261.61

631.81, 2+

5.00

None

Actin

AGFAGDDAPRAVFP

14

0.94

1389.67

695.84, 2+

5.29

None

FAGDDAPRAVFPSIVG

16

2.77

1617.81

809.91, 2+

5.74

None

AGFAGDDAPRAVFPSIVG

18

2.04

1745.87

873.94, 2+

5.86

None

AGFAGDDAPRAVFPSIVGRPRHQGIMVG

28

4.06

2877.47

720.37, 4+

5.49

None

EEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG

35

3.11

3519.65

1174.23, 3+

7.00

N-term Acetylation

MDEDDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVG

37

1.76

3824.73

1275.92, 3+

6.70

N-term Acetylation

-

VLIEKEPEPLPTVELTVSVPEEEPIVPKELRPEF

WNHF01003902.1.g54823 b

34

2.62

3881.09

971.28, 4+

6.36

None

Twitchin

VLIEKEPEPLPTVELT

16

4.55

1805.99

904.004, 2+

5.56

None

-

*YENKNIPQVCVGITAL

WNHF01002939.1.g23630 b

17

6.06

1760.90

881.46, 2+

5.95

None

Myophilin

FQVNDLYENKNIPQVCVGITALGRLAQKQPGYSGPIL

37

4.88

4043.11

1011.78, 4+

6.93

None

RLAQKQPGYSGPIL

14

3.21

1526.85

764.43, 2+

4.42

None

RLAQKQPGYSGPILGP

16

4.3

1680.92

841.47, 2+

4.38

None

RLAQKQPGYSGPILGPRE

18

2.5

1966.07

656.36, 3+

3.98

None

GDEVANNPSPMGMSRAVQNKIESKRSDSLE

30

2.94

3061.73

613.35, 5+

4.46

None

GDEVANNPSPMGMSRAVQNKIE

22

2.35

2385.10

1193.56, 2+

4.95

N-term Acetylation

AVNVIIP

7

2.49

724.45

725.45, 1+

5.44

None

GDEVANNPSPMGMSR

15

2.29

1602.67

802.34, 2+

4.34

N-term Acetylation

-

MNIEIKIPFTHPRVEY

WNHF01002824.1.g15025 b

16

3.43

2028.04

677.02, 3+

6.22

N-term Acetylation

Alpha-crystallin A

-

SAPPPLADESQITPTHLTPVK

WNHF01003902.1.g54819 b

21

3.61

2198.15

733.72, 3+

4.78

None

Twitchin

-

AYNRRVPFTHPFDDFPSHQWPAMRRPDPWEGFT

WNHF01003167.1.g35552 b

33

3.7

4098.90

820.79, 5+

6.45

None

Alpha-crystallin B

-

QPLRLEELLLSPGEADRLPLDSLTAPTTEEQTSYEKPGTIPLTGKVEATEKPADVF

WNHF01003902.1.g54825 b

56

3.28

6073.11

1215.63, 5+

7.00

Gln -> pyro-Glu

Twitchin

-

SAPAGAVDEAITPTSVGGVE

WNHF01002826.1.g15451 b

20

1.96

1826.89

914.45, 2+

4.88

None

Twitchin

  1. aQuery sequence generated from the work of Suwansa-ard et al., 2018, Peptides; bPredicted gene sequences from H. scabra genome assembly (GCA_026123075.1); cTranscript sequences generated by Ordoñez et al., 2021, Comp Biochem Physiol Part D.
  2. The putative annotations of known neuropeptide precursors were presented as they are reported in literature or appear in NCBI Databases for consistency.
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