Table 1 The sequence of primers detected by real time PCR.

From: Liraglutide combined with HIIT preserves contractile apparatus and blunts the progression of heart failure in diabetic cardiomyopathy rats

Gene

Sequence

Length (bp)

α-MHC

Forward: 5ʹ-AAGAAGAACTTGGTGCGGCT-3ʹ

234

Reverse: 5ʹ-ATCGTGCATTTTCTGCTTGGC-3ʹ

β-MHC

Forward: 5ʹ-GCACCGTGGACTACAATATCCT-3ʹ

117

Reverse: 5ʹ-TGGCAAACAGATTACTTAGGAGC-3ʹ

MURF1

Forward:5ʹ-GTGCCTACTTGCTCCTTGTGC-3ʹ

240

Reverse: 5ʹ-GGCGTAGAGGGCGTCAAACT-3ʹ

FOXO1

Forward: 5ʹ-CAGCCAGGCACCTCATAACA-3ʹ

85

Reverse: 5ʹ-GCCATTGACTGAAGAAGTTTGC-3ʹ

β-tublin

Forward: 5ʹ-CGAGAAGAATACCCCGACCG-3ʹ

115

Reverse: 5ʹ-CTACCAACTGGTGGACGGAC-3ʹ

  1. Myosin heavy chain-α (α-MHC), Myosin heavy chain- β(β-MHC), Muscle Ring Finger1 (MURF1), Forkhead Box Protein1 (FOXO1).
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