Fig. 2 | Scientific Reports

Fig. 2

From: TSC complex decrease the expression of mTOR by regulated miR-199b-3p

Fig. 2

Expression of TSC2 and phenotypes of the fibroblasts with or without TSC2-Q371fs, the WT was present as the average of all healthy volunteers. (A) Linear schematic of the protein structure of TSC2-WT, TSC2-Q371fs and the location of the primes and the qRT-PCR results, relative expression levels were normalized with GAPDH. (B) Immunofluorescence analysis using TSC2 (red) antibody and DAPI (blue) in Bar 15 μm. (C) The western blot of the endogenous TSC2 and the statistics, the originals gels can be seen in the supplementary figure S1. (D) Proliferation rate of fibroblasts. (E) Apoptosis assay of fibroblasts and the statistics.

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