Fig. 5

Activated Wnt/β-catenin signaling in NPCs decreases tip cell migration. Lateral still frames from time-lapse confocal microscopy of gfap:rtTA, TRE:β-cat*-EGFP larvae treated with indicated Dox concentrations. Snapshots are at 4-h intervals over 16 h of acquisition beginning at the onset of CNS angiogenesis (30 hpf). Magnified frames (4 × Zoom) highlight the hindbrain region bounded ventrally by the primordial hindbrain channels. Stereotypical tip cells are indicated by white asterisks. Stereotypical filipodia are indicated by red arrowheads. See supplementary videos 4, 5 for more detail.