Fig. 1 | Scientific Reports

Fig. 1

From: De novo assembly of the complete mitochondrial genomes of two Camellia-oil tree species reveals their multibranch conformation and evolutionary relationships

Fig. 1

Diagram of mitogenome assembly of C. oleifera and C. lanceoleosa. (A) Graphical fragment assembly (GFA) results of C. oleifera based on Flye assembler. (B) Validation of each linkage in the (C) oleifera GFA. (C) Graphical fragment assembly (GFA) results of C. lanceoleosa based on Flye assembler. (D) Validation of each linkage in the C. lanceoleosa GFA. Each sequence is represented by a node in the GFA, and detailed information for each node can be found in Table 1. Linkages between nodes represent the existence of overlapping regions. Verified regions are indicated above the sample wells of gel electrophoresis plot. For example, ‘c1c6’ represents PCR validation of the linkage between ctg1 and ctg6. ‘M’ stands for marker. Expected lengths (bp) of each PCR product are indicated below the electrophoresis plot. The full uncropped Gels image can be seen in Figs. S3 and S5. Our results confirm the presence of these linkages, indicating the diversity of genome structures. For C. oleifera, we randomly selected the path ctg8-ctg10-ctg12-ctg4-ctg12-ctg1-ctg6-ctg5-ctg2-ctg11-ctg7-ctg5-ctg3-ctg11-ctg9 to represent its complete mitogenome sequence. For C. lanceoleosa, we randomly used ctg1 and ctg5-ctg7-ctg4-ctg3-ctg2-ctg7-ctg6 to represent its complete mitogenome sequence.

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