Fig. 4
From: Identification of a novel TOP2B::AFF2 fusion gene in B-cell acute lymphoblastic leukemia
in vitro analysis. (A) Line graphs of the cell proliferation assay (n = 3, ****P < 0.0001). (B) Flow cytometric analysis for the apoptotic ratio of Ba/F3 cells. The early apoptotic cells were marked with positive Annexin V staining (APC-A) and negative 7-AAD staining. (C) Column graph of the early and late apoptosis ratio of Ba/F3 cells (n = 3, ***P < 0.001, *P < 0.05) (D) Western blot analysis of key proteins related to cell proliferation and apoptosis. (E) Representative flow cytometry histograms showing cell cycle distribution in Ba/F3 cells. (F) Western blot analysis of key proteins related to cell cycle. (G) Relative mRNA expression levels of early B cell differentiation markers in Ba/F3 cells. Gene expression was normalized to Gapdh. Data represent the mean ± SD of three independent experiments. The asterisks indicate non-specific bands or protein degradation products. Statistical analyses of all qPCR data and cell cycle assessments were performed using Student’s t-test, while CCK-8 results were analyzed using ANOVA.
