Fig. 6 | Scientific Reports

Fig. 6

From: Non-lethal sonodynamic therapy mitigates hypertensive renal fibrosis through the PI3K/AKT/mTORC1-autophagy pathway

Fig. 6

NL-SDT blocks TGF-β1-induced NRK-52E cells transformation.

(A-C) Protein levels of α-SMA, vimentin and collagen I measured by western blot. (D) NRK-52E cells transformation measured in cells stained with an antibody to α-SMA (red), and DAPI (blue: labels nuclei) after treatment. (E) NRK-52E cells transformation measured in cells stained with an antibody to E-cadherin (green), and DAPI (blue: labels nuclei) after treatment. n = 6, and experiments were performed independently in triplicate. ANOVA followed by the Tukey’s multiple comparison test was used for statistical analyses. *P < 0.05 vs. control; **P < 0.01 vs. control; ***P < 0.001 vs. control.

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