Fig. 2

Effect of AMLN diet and exercise on liver lipid deposition in mice. (a) Representative liver morphology. (b) H&E staining of mice liver tissue (scale bar = 20 μm). (c) Oil Red O staining of mice liver tissue (scale bar = 20 μm). (d–g) The analysis of MASLD activity score. (h) Liver lipid droplet area (%) as assessed by Image J. (i) Hepatic triglyceride content. All data are shown as means ± SEM, n = 6. * P < 0.05, ** P < 0.01, effect of diet; # P < 0.05, ## P < 0.01, effect of exercise. n = 6 is the number used for H&E, oil red O staining and TG analyses.