Fig. 5
Endothelial-CHRM3 contribute to high blood pressure. (a) Systolic, (b) Diastolic, and (c) MAP were monitored for three days in Chrm3f/f and VECre:Chrm3f/f male and female mice. (d) Averaged systolic, diastolic, and MAP were analyzed among Chrm3f/f and VECre:Chrm3f/f male and female mice. N = 16 male and female mice per genotype, a-c. repeated measures ANOVA with Bonferroni’s post hoc test, F(1,26) = 16.975, P < 0.001 (Systolic), F(1,26) = 8.774, P < 0.006 (Diastolic), and F(1,26) = 12.941, P < 0.001 (MAP) significantly different from corresponding values in Chrm3f/f mice. Individual data points (a-c) represent average BP measurements per 8 mice per day. Means are ± SEM. d. Unpaired two-tailed t-test, *P < 0.05 denotes significant difference between VECre:Chrm3f/f males and corresponding values in Chrm3f/f males, and *P < 0.05 denotes significant difference between VECre:Chrm3f/f females and corresponding values in Chrm3f/f females. Tail-cuff data points represent the average of approximately 15 BP measurements twice per day for three days per mouse. In a-d, red lines and bars represent females, and blue lines and bars represent males. Triangle signs and dotted bars represent knockout (VECre:Chrm3f/f) mice, and circle signs and clear bars represent control (Chrm3f/f) mice. Individual data points (d) represent average BP measurements per mouse.
