Table 1 Sequence of the primers used for genotyping.
Primer name | Primer sequence | Expected product size |
|---|---|---|
Chrm3flox/flox | F: 5’ GTTTAGCTGCCTGGAAGTTTGC 3’ R: 5’ TGTCTACATTTTCGTTCTGAGG 3’ | Floxed allele: 230 bp Wildtype allele: 200 bp |
VECre (Cdh5-Cre) | F: 5’ GAACCTGATGGACATGTTCAGGGA 3’ R: 5’ CAGAGTCATCCTTAGCGCCGTAAA 3’ | Cre Positive: 700 bp Cre Negative: no band |
