Fig. 6 | Scientific Reports

Fig. 6

From: Proteomic profiling identifies upregulation of aurora kinases causing resistance to taxane-type chemotherapy in triple negative breast cancer

Fig. 6

Aurora Kinases protein stability was increased by Paclitaxel treatment in TNBC cells. (A,B) IB analysis of indicated protein in MDA-MB-436 cells (A) and BT549 cells (B) treated with 50 µg/mL protein synthesis inhibitor cycloheximide (CHX) with or without 30nM or 300nM Paclitaxel for indicated time course. (C,D) IB analysis of indicated protein in BT549 cells treated with indicated compounds. (E) IB analysis of HA-tagged AURKB or HA-tagged AURKB-T232A protein expression in 293T cells after treatment with Paclitaxel at 300nM for indicated time course. (F) IB analysis of HA-tagged AURKB or HA-tagged AURKB-T232A protein expression in 293T cells after treatment with Paclitaxel at indicated dose for 24 h.

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