Fig. 9
Experimental verification of the expression of four prognostic genes and the functional role of AEBP1. (A) The mRNA expression levels of ATP2A3, PNMA1, AEBP1, and PLAT mRNA in liver cancer tissues and adjacent tissues. (B) Western blot analysis of ATP2A3, PNMA1, AEBP1, and PLAT protein expression levels in liver versus adjacent non-cancerous tissue. (C) CCK8 assayed cell viability after SiRNA-mediated target gene knockdown compared with control and negative control (NC) siRNA. (D) Transwell assays demonstrating the migration and invasion capabilities of HCCLM3 cells, with comparisons made between control, SiRNA-negative, and SiRNA-mediated target gene knockdown groups. Representative images of the invasion and invasion assays are shown on the left, while quantification of migrating and invading cells is displayed on the right.The p-value is displayed as: ns: not significant; *P < 0.05; **P < 0.01; ***P < 0.001.
