Fig. 1
Effect of Fer-1, DMF and erastin on PDE4B-OE plasmid induced changes in NP cells. (A) PDE4B suppressed the mRNA expression of Nrf2, and upregulated mRNA expression of ACSL4 and TFRC. Cells were transfected with PDE4B-OE plasmid for 24 h, later, cells were with erastin (10 µM), Fer-1 (1 µM), and DMF (100 µM) for 6 h. mRNA level of PDE4B, ACSL4, Nrf2 and TFRC were determined by qRTPCR. (B) Effect of Fer-1, DMF and erastin on cytokines level against PDE4B-OE plasmid in NP cells. Cells were transfected with PDE4B-OE plasmid for 24 h, later, cells were with erastin (10 µM), Fer-1 (1 µM), and DMF (100 µM) for 24 h. Cells supernatant was analysed by ELISA. (C–E) Effect of Fer-1, DMF and erastin on SOD, ROS and GPX4 level against PDE4B-OE plasmid in NP cells. Cells were transfected with PDE4B-OE plasmid for 24 h later cells were with erastin (10 µM), Fer-1 (1 µM), and DMF (100 µM) for 6 h. (C) Percentage of inhibition of SOD. (D) Percentage of ROS generation; (E) GPX4 concentration; Data shown are means ± SEM of n = 3. * p < 0.05, ** p < 0.01, ***p < 0.001, ****p < 0.0001.
