Fig. 4

HBV WGS using capture and amplicon based target enrichment. Top row: Relationship between viral load and percentage of reads on-target (i.e. HBV) for (A) probe-based capture sequenced on Illumina, (B) HEP-TILE sequenced on Nanopore and (C) HEP-TILE sequenced on Illumina. Bottom row: Relationship between viral load and percentage of genome with high confidence consensus for (D) probe-based capture sequenced on Illumina (x5 minimum depth), (E) HEP-TILE sequenced on Nanopore (x20 minimum depth) and (F) HEP-TILE sequenced on Illumina (x5 minimum depth). Points are coloured by the percentage genome coverage. Notes. Panels E and F show an outlier with viral load 5.41 log₁₀ IU/ml, with disproportionately 0% genome coverage. Repeat VL was 3 log₁₀ IU/ml. There is one sample, HEP-1529, in the lower left corner of Fig. 4B with 0.55% reads (260 reads) mapped to the HBV genome, which led to 45% coverage as shown in supplementary Table 2. This is mathematically possible as the minimum theoretical number of reads to obtain a whole genome at x20 depth in a 6 amplicon scheme is only 120 reads.