Fig. 3 | Scientific Reports

Fig. 3

From: Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells

Fig. 3

Analysis of HSA and Ada transient expression with different introns. At 48 h post-transfection, cells were digested and cultured in suspension with serum-free medium for about 7 days. Then supernatant were collected for further analysis. The expression was examined by Western blotting and the expression levels are expressed as relative changes by setting the values of the control vectors as 1. (A) Western blot analysis of HSA protein levels. (B) The HSA relative expression was analyzed by Image J. (C) Western blot results of Ada expression. (D) Ada expression normalized to that of the control. Error bars indicate the standard deviation of triplicate data.

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