Fig. 4 | Scientific Reports

Fig. 4

From: Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells

Fig. 4

Analysis of HSA and Ada stable expression levels. After establishing stable cell pools, cells were grown in fed-batch cultured with serum-free medium for about 12 days, and supernatant were collected for further analysis. (A) Western blot analysis of HSA expression levels. (B) Western bolt results of Adaexpression. (C) The analysisof HSA relative expression with Image J. (D) Ada expression normalized to that of the control. (E) ELISA analysis of HSA expression levels. (F) ELISA analysis of Ada expression levels. Error bars indicate the standard deviation of triplicate measurements (*P < 0.05).

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