Fig. 5 | Scientific Reports

Fig. 5

From: Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells

Fig. 5

Relative copy number and mRNA expression levels of gene of interest. Relative copy number and mRNA expression levels were calculated by the 2−ΔΔCt method and normalized to the control vector, whose value was set to 1. (A) Relative mRNA expression levels of HSA. (B) Relative mRNA expression of HC/LC in stable pools. (C) Relative copy number of HSA. (D) Relative copy number of HC/LC. Error bars indicate the standard deviation of triplicate measurements (*P < 0.05).

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