Table 1 Primers designed for DNA sequence amplification and probe construction for mapping of the 18S rDNA and RT domain of four C. eugenioides LTR-retrotransposons.
From: Chromosome distribution of four LTR retrotransposons and 18 S rDNA in coffea eugenioides
Sequence name | Primers forward and reverse | Tm (°C) |
|---|---|---|
18S rDNA | F: 5’- CTGCCAGTAGTCATATGC -3’ R: 5’- ATGGATCCTCGTTAAGGG -3’ | 60 |
Ty1/Copia Divo | F: 5’- GGCTCAAGTAATCTGGACGTATG -3’ R: 5’- CGGAACCGCTTTAATACCTTCT -3’ | 55 |
Ty1/Copia Nana | F: 5’- ACATTTGCACCAGTAACAAGAA -3’ 5’- TACCATCAGCTCTCCTTATGATATT -3’ | 55 |
Ty1/Copia Tork | F: 5’- TGGATCTGGAACTTGTCCAATTA -3’ R: 5’- CCCAATCTTCTATCTCTACTTATCTCC -3’ | 45 |
Ty3/Gypsy Athila | F: 5’- GTACAGGTGGTCCCGAAGAA -3’ R: 5’- TATCCCGTGCTCGACCATAAA -3’ | 45 |
