Fig. 2 | Scientific Reports

Fig. 2

From: Construction of a rodent neural network-skeletal muscle assembloid that simulate the postnatal development of spinal cord motor neuronal network

Fig. 2

The differentiation of SC-NSCs in the NN and NN-SkM assembloids. After 7 days of cultivation, the SC-NSCs differentiated into Map2-positive neurons (arrows in a and b), Olig2-positive oligodendrocytes (arrows in c and d) and GFAP-positive astrocytes (arrows in e and f) in the NN and NN-SkM assembloids. (g) Bar chart showing that the NN-SkM group had more Map2-positive cells and fewer GFAP-positive and Olig2-positive cells than the NN group (n = 6; **P < 0.01). (h) Heatmap representation of genes related to neural differentiation that were significantly differentially expressed (P < 0.05) between the NN-SkM, NN and SC-NSCs groups. Z-scoring label-free quantification (LFQ) intensities are depicted; red and blue represent increased and decreased values, respectively. Expression of genes associated with neuron differentiation (i), oligodendrocyte differentiation (j) and astrocyte differentiation (k). The results were significant across every group, with data input as values to the base 10 to allow log adjustment (n = 3, ***P < 0.001, **P < 0.01, *P < 0.05). The data are presented as means ± standard deviations (SD) in (g,i–k). Scale bars = 20 μm in (a–f).

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