Fig. 1 | Scientific Reports

Fig. 1

From: Mechanistic elucidation of human pancreatic acinar development using single-cell transcriptome analysis on a human iPSC differentiation model

Fig. 1

In vivo differentiation of hiPSC-derived pancreatic endoderm cells mimics early pancreatic development. (A) A schematic representation of induction of hiPSC-derived pancreatic endoderm cells and implantation into immunodeficient mice. A, activin A; C, CHIR99021; Y, Y-27632; K, KGF; N, Noggin; KC, KAAD-cyclopamine; TT, TTNPB; E, EGF. (B) Immunostaining of hiPSC-derived cells on Stage 4 Day 6 (total day 17) before implantation for PDX1 (green), NKX6.1 (red), and nuclei (blue). (C) Induction efficiency of hiPSC-derived PDX1+NKX6.1+ pancreatic endoderm cells on Stage 4 Day 6 (total day 17) before implantation, as evaluated by flow cytometry. Data from three independent experiments are presented as mean ± SD (n = 3). (D) Macroscopic images of grafts under the left renal capsule of a NOD/SCID mouse sacrificed 30 days after implantation (upper panel) and following isolation from the host mouse kidney (lower panel). Blue arrows indicate grafts. (E) Immunostaining of grafts on the indicated days after implantation for CPA1 (green) and NKX6.1 (red; upper panels), Mucin 1 (green) and E-CADHERIN (red; middle panels), and PRSS1 (green) and nuclei (blue; lower panels). Note that background signals were found in the host mouse kidney (MK). Scale bars: 100 μm in (B) and (E) and 5 mm in (D).

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