Fig. 2 | Scientific Reports

Fig. 2

From: Mechanistic elucidation of human pancreatic acinar development using single-cell transcriptome analysis on a human iPSC differentiation model

Fig. 2

Transition dynamics of differentiation states from pancreatic endoderm cells to pancreatic acinar cells revealed by scRNA-seq. (A) Clustering of cells using the tSNE method (left panel) and distribution of cells on the indicated days (right panels). Only the six clusters annotated as pancreatic cells (clusters 0, 1, 2, 8, 10, and 12) in Fig. S4 (n = 96 for pancreatic endoderm aggregates, n = 8 for day 11 grafts, and n = 5 for day 25 grafts) were reclustered. (B) Distribution of cells expressing marker genes for pancreatic endoderm cells (PDX1, NKX6.1, PTF1A, and SOX9), pancreatic endocrine cells (CHGA, INS, and GCG), and pancreatic acinar cells (PRSS1, SPINK1, CPA1, CPA2, CPB1, and CTRC). (C) The direction and magnitude of differentiation stage transition among cell populations by RNA velocity. The lower boxed panel shows the velocity map by selecting clusters 5, 11, and 12. (D) Visualizing the differentiation stage transition from pancreatic endoderm to acinar lineage cells (upper panel) and changes in the expression of pancreatic acinar marker genes during the differentiation process (lower panel) by pseudotime analysis.

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