Fig. 2
The clustering and identification of cell types in mouse kidney subjected to ischemia-reperfusion injury. (A) The distribution of mouse IRI kidney cells in distinct sample timepoints. The data were collected at time points of 4 h, 12 h, 2 days, 14 days, and 6 weeks following ischemia-reperfusion injury (IRI) in mice compared to the Sham control group (with 4 replicates per time point). (B) UMAP plots of all datasets integrated with Harmony. (C) The proportion of cell types. (D) Expression patterns of cluster-enriched markers. (E) Alterations in the proportions of five cell clusters in PT. ATL, thin ascending limb of loop of Henle; CNT, connecting tubule; CTAL, thick ascending limb of loop of Henle in cortex; DCT, distal convoluted tubule; DTL, descending limb of loop of Henle; EC, endothelial cells; Fib, fibroblasts; ICA, type A intercalated cells of collecting duct; ICB, type B intercalated cells of collecting duct; Macro, macrophages; MTAL, thick ascending limb of loop of Henle in medulla; New PT1/2/3, new proximal tubule (PT) clusters 1/2/3; PC, principle cells of collecting duct; Pod, podocytes; PT S1/S2/S3, S1/S2/S3 segment of proximal tubule; Uro, urothelium.
