Fig. 1

Effect of actinomycin D on the expression of selected genes. (A) Relative quantity of Actb, Sdha, Ppia, H2afz and B2m transcripts in blastocysts. (B) Relative quantity of Bbc3/PUMA, Bax, Bak1, Bcl2, and Bcl2l1 transcripts in blastocysts. The values are arithmetic means + SEMs; Cont, control blastocysts; ActD, actinomycin D-treated blastocysts; n = 5. Statistical differences between the groups were assessed via the unpaired T-test (*P < 0.05), (**P < 0.01), (***P < 0.001).