Table 12 Analysis of co-localisation of apoptotic cells and acidified phagosomes in human trophoblast cell line Ac-1M88 treated with bafilomycin A1 for 24 h.

	Control	Bafilom.	Bafilom.	Bafilom.	Bafilom.	Bafilom.
	Control	(0.01 nM)	(0.1 nM)	(0.2 nM)	(0.5 nM)	(2 nM)
Number of evaluated optical fields	76	50	51	51	26	25
Total number of evaluated TB cells	5479	3603	3555	3229	2143	1487
Mean number of TB cells per optical field (± S.E.M.)	72.05 ± 3.11^a	72.06 ± 3.34^ab	69.71 ± 2.81^ab	63.31 ± 2.82^ab	82.42 ± 6.62^a	59.48 ± 3.10^ab
Total number of apoptotic cells in TB	47	37	56	57	47	162
Mean number of AC per optical field (± S.E.M.)	0.62 ± 0.12^a	0.74 ± 0.21^ab	1.10 ± 0.24^ab	1.12 ± 0.24^ab	1.81 ± 0.42^b	6.48 ± 0.64^c
Average % of LT + TB AC (± S.E.M.)	0.92 ± 0.25^a	0.86 ± 0.25^a	1.36 ± 0.30^a	1.65 ± 0.35^a	1.46 ± 0.34^a	8.31 ± 0.67^b
Average % of LT- TB AC (± S.E.M.)	0.14 ± 0.05^a	0.17 ± 0.10^a	0.26 ± 0.13^a	0.17 ± 0.10^a	0.30 ± 0.13^a	2.57 ± 0.66^b
Proportion of LT + /LT- AC (%)	82.98:17.02	83.78:16.22	85.71:14.29	89.47:10.53	82.98:17.02	74.69:25.31

Results are expressed as mean ± S.E.M. or medians with interquartile range; AC, apoptotic cells; TB, trophoblast; LT, LysoTracker. Statistical analysis: Mean number of cells per optical field, ANOVA (P = 0.0061), followed by Tukey’s test; Mean number of apoptotic cells per optical field, ANOVA (P < 0.0001), followed by Tukey’s test; Mean number of LT + apoptotic cells per optical field, ANOVA (P < 0.0001), followed by Tukey’s test; Average % of LT + apoptotic cells, ANOVA (P < 0.0001), followed by Tukey’s test; Mean number of LT- apoptotic cells per optical field, ANOVA (P < 0.0001), followed by Tukey’s test; Average % of LT- apoptotic cells, ANOVA (P < 0.0001), followed by Tukey’s test; Proportion of LT + /LT- apoptotic cells (%),Chi-squared test with five degrees of freedom (P = 0.15), Fisher test for each pair of columns.
^a no significance, ^b significance towards "a", resp."c", ^c significance towards "a" and "b".

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