Fig. 1

CBD repressed viability of OC cells by a CBR1-dependent mechanism. (A) OC cells were pre-incubated with the respective receptor antagonist AM251 or AM631 for 30 min at a final concentration of 100 µM and then further co-incubated with CBD (40 µM) for another 24 h. The cell inhibition rate was detected by CCK-8 assay. (B,C) Western blot analysis was conducted to detect CB1R expression in SKOV3, Hey-A8, and ES-2 cells. (D) The ES-2 cells were treated with gradient concentrations of CBD (10 µM-60 µM) for 24 and 48 h, respectively, and the cell viability was assessed by CCK-8 assay. DMSO was used as a negative control. Each value indicates the mean ± SD of results obtained from three independent experiments. *p < 0.05, **p < 0.01, *** p < 0.001.