Fig. 2 | Scientific Reports

Fig. 2

From: Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury

Fig. 2

Effects of hypothermia on mitophagy and apoptosis in model of OGD/R-induced BV2 mouse microglia cells damage. (A) The representative electron micrographs of mitochondria were observed by TEM. Red arrow indicated mitophagy-like event, double yellow arrow represented severe broken mitochondria, yellow arrow represented severe broken mitochondria, blue arrow represented normal mitochondria. Scale bar = 500 nm. (B-J) The western bands of LC3B I, LC3B II, cleaved caspase 3, caspase3, β-actin and GAPDH, as well as the quantitative analysis. (K-L) The mRNA expression of LC3B and the quantitative analysis. Data was presented as the mean ± SD (n ≥ 3). * P < 0.05, vs. Sham group; # P < 0.05, vs. OGD group; & P < 0.05, vs. OGD/R-NormoT-2 h groups; $ P < 0.05, vs. OGD/R-NormoT-4 h group.

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