Fig. 4
σX contributes to biofilm formation in B. velezensis 118. Representative images show the pellicle formation in LBGM medium (A) and colony architecture morphology on LBGM plates (B) of B. velezensis 118 (WT) and its derived sigX::erm mutant after 1, 3, or 5 d of incubation at 30 °C. (C) Biofilm production was quantified for both 118 WT and the sigX::erm mutant grown in LBGM medium at various indicated timepoints. Data represent the mean values ± SD (n = 3). Statistical significance was determined by a student t-test, **P < 0.01. (D) Representative images show the pellicle formation of B. subtilis 3610, its derived sigX null mutant, and the complement strain sigX PspacsigX (sigX PsigX) in LBGM medium (top) and colony architecture morphology on LBGM plates (bottom) after 15–24 h incubation at 37 °C. Scale bars, 5 mm.
