Fig. 1 | Scientific Reports

Fig. 1

From: Human type I interferons protect Vero E6 and ARPE-19 cells against West Nile virus and are neutralized by pathogenic autoantibodies

Fig. 1

Cytopathic effect of WNV infection in the presence of Type I IFNs. The rate of WNV infection was evaluated by measuring virus-induced cytopathic effects (CPE) in ARPE-19 cells (A) and Vero E6 cells (B) at 0, 24, and 48 h post-infection. Cells were pre-treated overnight with type I IFNs (IFN-β, IFN- ω, and IFN- α2) at concentrations of 10 ng/mL, 1 ng/mL, 100 pg/mL, 50 pg/mL, and 10 pg/mL, followed by infection with 100 TCID50 of WNV. In ARPE-19 cells, all type I IFNs effectively restricted WNV replication at concentrations as low as 50 pg/mL or higher. In contrast, in Vero E6 cells, IFN-α2 and IFN-β provided protection at concentrations of 100 pg/mL or higher, while IFN-ω was protective only at 10 ng/mL. All experiments were performed in triplicate. The figures have been simplified to display the most relevant time points and virus inoculum. Full data, including additional time points and concentrations, are available in the supplementary materials.

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