Fig. 5 | Scientific Reports

Fig. 5

From: Human type I interferons protect Vero E6 and ARPE-19 cells against West Nile virus and are neutralized by pathogenic autoantibodies

Fig. 5

Blocking of IFN-ω activity utilizing sera from patients with WNV neuroinvasive disease. The rate of WNV infection, evaluated through virus-induced cytopathic effects 24 h post-infection in ARPE-19 cells treated with different concentrations of IFN-ω (10,000, 500, 100, and 50 pg/mL), was examined. Despite the presence of IFN-ω, increased WNV replication was observed when ARPE-19 cells were treated with all tested IFN-ω concentrations in conjunction with sera from patients with neuroinvasive WNV disease and neutralizing autoantibodies (IFN-I-NTAbs) against IFN-ω (n = 4). At 10,000 pg/mL, the neutralizing effect of these autoantibodies diminished but was not completely abolished, as the protective activity of IFN-ω was only partially restored. This effect was compared to treatment with sera from patients with WNV disease without IFN-I-NTAbs (n = 1). All sera were tested at a single dilution of 1:10. All experiments were performed in triplicate.

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