Fig. 7 | Scientific Reports

Fig. 7

From: Human type I interferons protect Vero E6 and ARPE-19 cells against West Nile virus and are neutralized by pathogenic autoantibodies

Fig. 7

Correlation between IFN-I-NTAbs ARPE assay and ELISA/luciferase assays for neutralizing antibodies against type I IFNs. Correlation between the IFN-I-NTAbs ARPE cell-based assay and the ELISA and luciferase-based assays for detecting neutralizing antibodies against IFN-β (A), IFN-ω (B), and IFN-α2 (C). The Y-axis shows the percentage neutralization value of the IFN-I-NTAbs ARPE assay at different concentrations of type I IFNs (50 pg/mL, 100 pg/mL, 500 pg/mL, and 10 ng/mL). Red triangles represent positive results from the luciferase assay at 10 ng/mL. Green circles indicate positive results from the luciferase assay at 100 pg/mL. Black squares represent samples that were negative in the luciferase assay at 10 ng/mL but may neutralize at lower concentrations, as shown in the ARPE-NT assay. Purple diamonds indicate positive results from the ELISA assay. These data demonstrate the concordance between the newly developed IFN-I-NTAbs ARPE assay and established methods, highlighting the ability of the ARPE assay to detect and quantify the functional neutralizing activity of type I IFN auto-antibodies, even at varying IFN concentrations.

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