Table 1 The primer sequences of related genes used in qRT-PCR.

From: Establishment and characterization of three gemcitabine-resistant human intrahepatic cholangiocarcinoma cell lines

Genes

Forward (5’- to 3’-)

Reverse (5’- to 3’-)

β-actin

CACCATTGGCAATGAGCGGTTC

AGGTCTTTGCGGATGTCCACGT

E-cadherin

GCCTCCTGAAAAGAGAGTGGAAG

TGGCAGTGTCTCTCCAAATCCG

N-cadherin

CCTCCAGAGTTTACTGCCATGAC

GTAGGATCTCCGCCACTGATTC

Claudin-1

GTCTTTGACTCCTTGCTGAATCTG

CACCTCATCGTCTTCCAAGCAC

β-catenin

CACAAGCAGAGTGCTGAAGGTG

GATTCCTGAGAGTCCAAAGACAG

Occludin

ATGGCAAAGTGAATGACAAGCGG

CTGTAACGAGGCTGCCTGAAGT

Slug

ATCTGCGGCAAGGCGTTTTCCA

GAGCCCTCAGATTTGACCTGTC

Snail

TGCCCTCAAGATGCACATCCGA

GGGACAGGAGAAGGGCTTCTC

Zo-1

GTCCAGAATCTCGGAAAAGTGCC

CTTTCAGCGCACCATACCAACC

Vimentin

AGGCAAAGCAGGAGTCCACTGA

ATCTGGCGTTCCAGGGACTCAT

ZEB1

GGCATACACCTACTCAACTACGG

TGGGCGGTGTAGAATCAGAGTC

PI3K

GAAGCACCTGAATAGGCAAGTCG

GAGCATCCATGAAATCTGGTCGC

Akt

TGGACTACCTGCACTCGGAGAA

GTGCCGCAAAAGGTCTTCATGG

IGF-1R

CCTGCACAACTCCATCTTCGTG

CGGTGATGTTGTAGGTGTCTGC

p53R2

ACTTCATCTCTCACATCTTAGCCT

AAACAGCGAGCCTCTGGAACCT

Bcl-2

ATCGCCCTGTGGATGACTGAGT

GCCAGGAGAAATCAAACAGAGGC

Bax

TCAGGATGCGTCCACCAAGAAG

TGTGTCCACGGCGGCAATCATC

SOD2

CTGGACAAACCTCAGCCCTAAC

AACCTGAGCCTTGGACACCAAC

Back to article page