Fig. 3 | Scientific Reports

Fig. 3

From: PPM1B degradation mediated by TRIM25 ubiquitination modulates cell cycle and promotes gastric cancer growth

Fig. 3

PPM1B-mediated dephosphorylation of CDK2 inhibits cell proliferation. (A) Analysis of the cell cycle distribution of AGS and MGC-803 cells overexpressing PPM1B and mock control plasmids by flow cytometry. (B) The protein levels of overexpressed PPM1B GC cell cycle-related markers, such as p-CDK2, CDK2, CDK4, Cyclin D1, Cyclin E1, were detected by WB. (C, E) Flow cytometry analysis of the cell cycle distribution of AGS and MGC-803 cells with knockdown, knockout PPM1B and mock control plasmids. (D, F) The protein levels of cell cycle-related markers of knockdown and knockout PPM1B GC cells were detected by WB.

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