Table 2 Primers and related information of the target gene and the candidate reference genes.

From: State of thermal tolerance in an endangered himalayan fish Tor putitora revealed by expression modulation in environmental stress related genes

Gene

Primer pairs (5′-3′)

Amplification efficiency (%)

GenBank accession no.

TpHSP90β

F: GGAGAAGAAGATGCCCTGGAAC

R: GCTTGATGTTGACGACGCTCT

99

OQ928015.1

TpHSP70

F: ATCACCATCACCAACGACAAG

R: TTTCCTTTCAGGTTCTCATCTTCC

100

OQ928014.1

TpHSP60

F: GCAGCCCTAAAGTCACCAAAG

R: CCATCTCCAGCCTCCTCATT

104.6

OQ928013.1

TpHSP30

F: CGGCTTTGCTTTGACGCT

R: CCCTTCCCATCCTCTTGCTT

103.3

OQ928011.1

TpHSP20

F: CGACTTGCCTCCTTCTCTTC

R: GGTCCAACTTTCCCATTCCA

105.4

OQ928010.1

TpHSP47

F: TAAGGTCAGCGTGGAGGTCA

R: ATGGCAGAGGCGTGGAATAC

104.8

OQ928012.1

TpCDKN1B

F: TCAAAGCAAACGCTCACACATC

R: TCAACTTCAGCCCTCATCGG

99.7

XM_026238549

TpWAP65-1

F: AGAGCACTGATGACCACATTGA

R: GAGAAGACTGCATCCACTTCAC

104.2

ON156522.1

TpTBP

F: TCGTCCCTCAGTTGCAGAAT

R: CTGGCTCGAAGTGCTATCGT

99.60

OP377748.1

TpRPS18

F: GACGGAAGACGCAAAATCGC

R: CACCACCCTCTCCACCTCATC

105.10

OP377749.1