Fig. 5
From: Intratumoral administration of mRNA COVID-19 vaccine delays melanoma growth in mice
IT injection of mRNA-1273 does not alter cytokines or have transcriptional effects. (A) Cytokine levels of tumor, inguinal lymph node, and sera at 48 h after IT injection with 3 µg mRNA-1273 (mRNA) or PBS. Heat map shown depicts mean pg/ml of each cytokine log10 transformed (n = 5 mice/group, n = 10 mice total). tSNE plot of (B) immune cell types separated by treatment groups and (C) the cells defined by their single-cell transcriptome analysis from combined mRNA-1273 and PBS IT injections. (D) The proportions of cell projections in tumors treated with mRNA-1273 or PBS IT injections. (B–D) scRNAseq analysis was performed using pooled samples (n = 5 mice/group, n = 10 mice total), prior to library preparation.
