Fig. 5
From: Extracellular vesicles efficiently deliver survival motor neuron protein to cells in culture
Application of conditioned medium containing EVs loaded with 3F-SMN protein onto A549:HA-Gemin2 cells results in FLAG-positive nuclear foci. Panel A: Conditioned medium (CM) from HepG2 cells infected with Ad3F-SMN or mock-infected was applied to A549:HA-Gemin2 cells in the presence or absence of 100 μM 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB). Two h post-treatment, the cells were washed and processed for immunofluorescent staining with anti-FLAG and anti-HA, and images were captured by epifluorescence microscopy. Arrows indicate representative nuclear gem-like foci. Scale bars represent 10 µm. Panel B: FLAG-positive nuclear foci were quantified and expressed per 100 cells. Significance was calculated after transforming data by log transformation using two-way ANOVA followed by Tukey’s multiple comparisons test. Four asterisk (****) indicates p < 0.0001. Data represents 3 independent experiments.
