Fig. 6
From: Extracellular vesicles efficiently deliver survival motor neuron protein to cells in culture
EV-delivered 3F-SMN protein is internalized by recipient A549:HA-Gemin2 cells and localizes in gem-like structures. Panel A: Confocal immunofluorescence images of A549:HA-Gemin2 cells that were treated for 2 h with mock or 3F-SMN CM, and processed with antibodies to HA and FLAG. Panel B: Images in panel A were examined through orthogonal projections generated from z-stacks. Panel C: Orthogonal projections generated of z-stacks of parental A549 cells stained with antibodies to tubulin and SMN. Scale bars represent 10 µm.
