Fig. 3 | Scientific Reports

Fig. 3

From: METTL3 promotes the progression of non-alcoholic fatty liver disease by mediating m6A methylation of FAS

Fig. 3

Knockdown of METTL3 inhibits FAA-induced hepatocyte damage. (A) The expression of METTL3 was measured using qPCR in sh-NC and sh-METTL3 transfected cells. Primary hepatocytes were exposed to FFA and transfected with shRNAs, and (B) cell viability was measured using CCK-8; (C) apoptosis was assessed using flow cytometry and (D) apoptosis rate was quantified; (E) lipid was observed using Oil Red O staining assay; (F) mRNA expression of SCD1, SREBP1, and FAS were detected using qPCR; (G) protein levels of ACAT2, HMGCR, SCD1, SREBP1, and FAS were measured using western blotting. n = 3.

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