Fig. 4 | Scientific Reports

Fig. 4

From: METTL3 promotes the progression of non-alcoholic fatty liver disease by mediating m6A methylation of FAS

Fig. 4

METTL3 promotes m6A methylation of FAS. (A) METTL3 expression was detected using qPCR following pcDNA3.1-NC and pcDNA3.1-METTL3 transfection. (B) Effect of METTL3 on m6A levels was assessed using m6A dot blot. (C) The m6A expression of ACAT2, HMGCR, SCD1, SREBP1, and FAS mediated by METTL3 was measured using MeRIP. (D) RIP was used to determine the interaction between METTL3 and FAS. (E) Potential m6A methylation sites in FAS were predicted. (F) The sequences of all potential modifing sites. Dual-luciferase reporter assay revealed the combined relationship between FAS containing (G) site 1 or (H) site 2 and METTL3. (I) Effect of METTL3 on the stability of FAS. n = 3. NS no significance.

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