Fig. 9

Validation of ETV4 expression and its impact on the proliferation, migration, and invasion of nasopharyngeal carcinoma cell. Note: (A) The expression levels of ETV4 mRNA and protein in nasopharyngeal carcinoma cell lines CNE1 and CNE2; (B) The knockdown efficiency of ETV4 by three siRNAs assessed using quantitative real-time PCR (qRT-PCR) and Western blotting; (C) CCK-8 assay was used to evaluate cell proliferation; (D) Colony formation assay was conducted to assess the proliferation/cloning ability; (E) Wound healing assay was performed to determine cell migration capability; (F)-(G) Transwell migration and invasion assay were used to evaluate the migratory and invasive capabilities of cell, respectively.