Fig. 2

Bioinformatic analyses identified differentially expressed genes in COAD. Volcano plots were visualized to verify differentially expressed genes in (A) COAD tissues compared to normal tissues based on TCGA RNA-seq and (B) metformin-treated COAD cells in contrast to untreated COAD based on GSE67342 (GEO database). (C) Venn diagram was used to identify significant target genes, and there were 6 common genes between TCGA RNA-seq data and GSE67342. Among them, FJX1, which plays an important role in COAD, was selected as the target gene and highlighted in red. (D) Biological process data from gene ontology analysis using ShinyGO demonstrated that FJX1 is involved in organ development and cell signaling. (E) The construction of survival curves was based on the trisection of FJX1 expression levels for patients with COAD, utilizing the OncoLnc. The patients with high expression levels of FJX1 were depicted in a red line and those with low expression levels in a black line. (F) Expression of FJX1 in several cancers from TCGA was analyzed using the TIMER2.0 database, which revealed that FJX1 was upregulated in most cancers.