Fig. 2 | Scientific Reports

Fig. 2

From: Esketamine induces embryonic and cardiac malformation through regulating the nkx2.5 and gata4 in zebrafish

Fig. 2

EK exposure (0.21, 0.42 and 0.84 mM) induces embryonic dysplasia with pericardial and SV-BA distance (Fig. 2C). (A) (Table 5 suppl.), Pericardial area at 72 hpf of four randomly chosen zebrafish embryos, there was no pericardium swelling in the control and 0.21 mM groups (P > 0.05), the embryos both in 0.42 and 0.84 mM groups were pericardium swelling (rate = 100%, P < 0.01). (B) (Table 6 suppl.), SV-BA distance of four randomly chosen zebrafish embryos at 72 hpf. C, Images of zebrafish at 72 hpf under a stereo microscope; scale bar: 200 μm; magnification: × 6.25. Each group (except 0.84 mM) contains 30 zebrafish embryos and all reperformed for three independent experiments. All data were analyzed using one-way ANOVA with Bonferroni correction. A, bulbar artery; V, sinus venous. **P < 0.01, ***P < 0.001, ****P < 0.0001.

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